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CHROMAFIL disposable syringe filters are used for filtration of suspended matter from liquid samples (1–100 mL).
The filter can be used for sample preparation for HPLC, GC, ICP, AAS, TOC, DOC, IR, NMR, photometry, and spectroscopy samples to filter out contaminants and extraneous particles that can affect analytical results or clog liquid pathways, frits, etc.
CHROMAFIL syringe filters are HPLC and UPLC certified.
A certificate of analysis (COA) is available upon request.
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- Different membrane types to meet multiple filtration applications
- Low extractable, sonic welded, leak-free polypropylene housings
- Fast flow geometry for minimized back pressure during filtration
- Very low hold-up volumes for maximum volume recovery
- Certified clean for HPLC and UPLC analysis
- Standard Luer-Lok™ inlet and Luer slip outlet
- Designed to be compatible for use on all common automated filtration systems,
e.g. SOTAX® dissolution systems
Recommended filter size depending on sample volume:
| Sample Volume | Recommended Filter Diameter | Filter Hold-up Volume (approx.) | Available Filtration Area |
|---|---|---|---|
| 1 ml | 3 mm | 0.5 µL | 0.07 cm2 |
| 1 - 5 ml | 15 mm | 12 µL | 1.33 cm2 |
| 5 - 100 ml | 25 mm | 120 µL | 4.91 cm2 |
Syringe Filters Feature:
- Polypropylene Housing
Better solvent stability compared to acrylate and polystrene filters - Shells ultrasonically sealed, not glued
No extractable components from glues - Filtration in both directions possible
The liquid cannot bypass the membranes. - Luer-lok on side of entry
Safe connection on the “high pressure” side - Luer exit
Standard luer for 3 mm and 25 mm filters, minispike luer with low dead volume and small OD for 15 mm filters - Deflector
The stream of liquid is broken and distributed, and does not directly hit the membrane: this prevents rupture of the membrane - Star-shaped distribution device
The liquid is evenly distributed to the whole membrane surface: This results in a better utilization of the total area. The filter is not plugged up rapidly, providing high flow efficiency. - Color Coded
Filter with 0.2 µm pores have a yellow upper shell and filters with 0.45 µm pores are colorless. The different membrane types are distinguished by different colors. - Low Dead Volume
~120 µl for 25 mm Ø, 12 µl for 15 mm Ø, 5 µl for 3 mm Ø
Housing Material (Polypropylene)
- Lowest Content of Extractable Substances
This low content is due to high-density polypropylene housing. In comparison to filters from polycarbonate, polyacrylate, or polystyrene, all CHROMAFIL® filters are resistant to nearly all organic solvents (See the Chemical Compatibility Table). - Ultrasonically Sealed
The filters are welded, not glued, because glue may have extractable ingredients. Welding leads to a tight connection between both parts, thus the filter can be used in both directions. No fluid can bypass the membrane.
- Special Thick Rim
The thick housing rim is ideal for use in laboratory robots (e. g. SOTAX®, Benchmate™).
Luer Connections
- Luer-lok on the side of the entry
For a safe connection on the high-pressure side, every filter provides a side-entry lure lock. - Luer exit
For 25 and 3 mm filters: standard luer exit. For 15 mm filters: mini spike – This luer configuration offers a low hold-up volume and easy filtration into autosampler vials and NMR tubes. Filter inlet and filter exit can be fitted to all CHROMABOND® columns and accessories for selective sample preparation with the aid of a special adaptor.
“Crash Plate”/Deflector
- No rupture of the membrane due to the “crash” plate
The input solvent stream is “broken” and distributed by the crash plate and does not directly hit the membrane: this prevents the rupture of the membrane by diverting streams into four lanes.
Distributor In “Star” Form
- Optimum flow geometry because of the star shaped distribution device
The stream of liquid is broken into 4 lanes by the impact plate and then further distributed to 8 slots in the form of a star connected with 5 or 8 circular channels (for 15 mm and 25 mm filters, respectively). Thus, the fluid is able to penetrate the membrane on the whole surface, not only on a small region. The filter is not plugged up rapidly, which results in high flow efficiency.
Membranes
- Different Membrane Materials
| Membranes | |
|---|---|
| Polyester | PET (with or without glass fiber pre-filter) |
| Regenerated Cellulose | RC |
| Teflon | PTFE |
| Cellulose Mixed Esters | MV |
| Cellulose Acetate | CA |
| Polyamide | PA |
| Polyethersulfone | PES |
| Polyvinylidene diflouride | PVDF |
| Glass Fiber | GF |
For optimal filtration results using CHROMAFIL® filter consider the following recommendations:
- We recommend either discarding the first 1 ml or rinsing the filter unit with 1 ml of primary solvent before sample filtration
- Before filling the syringe, draw approx. 1 ml air into it. This air helps to minimize the remaining fluid in the filter.
- Start with gentle pressure at the beginning of the filtration. This helps assure maximum throughput. As the filter “accumulates” particles, filtration become more difficult and the pressure will increase on the filter.
- Change filter when resistance becomes excessive in order to avoid housing rupture.
- Do not use CHROMAFIL® syringe filters for direct patient care applications; they are designed for laboratory use only!
- Do not use syringes smaller than 10 ml; the pressure generated may exceed the 6 bar limit of the filter.
- Do not use at temperatures above 55°C (131°F).
- Do not reuse the filter.
CHROMAFIL Disposable Filters
This table lists the chemical compatibility of our CHROMAFIL materials. The chemical compatibility depends on several parameters such as time, pressure and concentration. In most cases, CHROMAFIL filters will have only short contact with a solvent. In these cases they may be used despite limited compatibility. For example, a PTFE filter with PP housing does not liberate any UV-detectable substances during filtration of 5 ml THF, although PP shows only limited resistance towards THF.
INTERACTIVE COMPATIBILITY CALCULATOR
+ Resistant | – Not Resistant | O Limited Resistance
- MV-Cellulose Mixed Esters
- CA-Cellulose Acetate
- RC-Regenerated Cellulose
- PA-Polyamide
- PTFE-Polytetrafluoroethylene (Teflon)
- PVDF-Polyvinylidene difluoride
- PES-Polyethersulfone
- PET-Polyester
- GF-Glass Fiber
- PP-Polypropylene (Housing Material)
Solvent
Material
MV
CA
RC
PA
PTFE
PVDF
PES
PET
GF
PP
Acetaldehyde
-
-
+
0
+
+
+
+
0
Acetic acid, 100%
-
-
-
-
+
+
+
+
+
+
Acetone
-
-
+
+
+
+
+
+
+
+
Acetonitirle
-
-
+
+
+
+
+
+
+
+
Ammonia, 25%
-
-
0
-
+
+
+
0
+
+
Benzene
+
+
+
+
+
0
+
+
0
n-Butanol
+
+
+
0
+
+
+
+
+
+
Cyclohexane
+
+
+
0
+
+
+
+
+
+
Dichloromethane
+
-
+
-
+
+
-
+
+
-
Diethyl ether
0
0
+
+
+
+
+
+
+
0
Dimethylformamide
-
-
0
+
+
-
-
+
+
+
1, 4-Dioxane
-
-
+
+
+
0
-
+
+
0
Ethanol
-
+
+
+
+
+
+
+
+
+
Ethyl acetate
-
-
+
+
+
+
+
+
+
0
Ethylene glycol
0
0
+
+
+
+
+
+
+
+
Formic acid, 100%
+
-
0
-
+
+
+
0
+
+
Hydrochloric acid, 30%
-
-
-
-
+
+
+
-
+
+
Methanol
-
-
+
+
+
+
+
+
+
+
Nitric acid, 65%
-
-
-
-
0
0
0
+
-
Oxalic acid, 10% aqueous
+
-
+
-
+
+
+
+
+
Petroleum ether
+
+
+
+
+
+
+
+
+
+
Phosphoric acid, 80%
-
-
0
-
+
0
+
+
+
Potassium hydroxide, 1 mol/1
-
-
0
+
+
0
+
0
+
+
2-Propanol
+
+
+
+
+
+
+
+
+
+
Sodium hydroxide, 1 mol/1
-
-
0
+
+
0
0
0
0
-
Tetrachloromethan
+
-
+
+
+
0
+
+
0
Tetrahydrofuran
-
-
+
0
+
+
-
+
+
-
Toluen
+
-
+
+
+
+
+
+
+
-
Trichloroethylene
+
+
+
0
+
+
+
+
0
Trichloromethan
+
-
+
-
+
+
-
+
+
-
Urea
+
+
+
+
+
+
+
+
+
Water
+
+
+
+
+
+
+
+
+
+
DATA NOT GUARANTEED